Glucose can be anearobically decomposed into lactic acid and ethanol. This decomposing process is called as glucolytic pathway. In this pathway, some products such as glucose-1-phosphate, glucose-6-phosphate, fructose-6-phosphate, fructose-1,6-bisphosphate, etc. are produced as intermediary products. Here, glucose-1-phosphate, fructose-6-phosphate and glucose-6-phosphate are analyzed using Asahipak NH2P-50 4E by ion-exchange mode. It was confirmed that the retention is stronger and separation is better by using lower concentration phosphate buffer.

Sample : (Left); 20mg/mL each, (Right); 10mg/mL each, 20µL
1. Glucose-1-phosphate
2. Fructose-6-phosphate
3. Glucose-6-phosphate

Column       : Shodex Asahipak NH2P-50 4E (4.6mmID*250mm)	
Eluent       : (Left); 10mM Sodium phosphate buffer(pH4.4)
             : (Right); 150mM Sodium phosphate buffer(pH4.4)
Flow rate    : 1.0mL/min			
Detector     : Shodex RI			
Column temp. : 40°C